Isolation, Identification and 3D Structure of Extracellular Lipase from Mucor hiemalis by Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Subcutaneous zygomycosis caused by Mucor hiemalis in an immunocompetent patient.
Zygomycosis is an opportunistic fungal infection with a high mortality rate. It is known to cause invasive disease in immunocompromised hosts but it may produce only cutaneous/ subcutaneous infections in immunocompetent hosts. Treatment is difficult due to its fulminant course and lack of effective anti-fungal drugs. Here, we report a rare case of subcutaneous zygomycosis caused by Mucor hiemal...
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Objective Fungi produce many different carotenoids and some are attractive in medical and industrial sources. In this work the ability of Mucor hiemalis (PTCC 5292) to produce carotenoids in media with different nitrogenous and carbon sources and incubation with white, yellow, blue and red lights (15W, 220V, E27) against dark-grown were studied. Materials and Method The microorganism cultiva...
متن کاملIdentification by Two-Dimensional Gel Electrophoresis
We have previously identified proteins in fractions of culture filtrate ofMycobacterium tuberculosis with the capacity to induce cytokine production in monocytes, by using a technique we defined as "monocyte Western blotting" (immunoblotting). In this series of experiments, we have extended this technique to two-dimensional gel electrophoresis and have identified a novel 58-kDa protein ofM. tub...
متن کاملLipase of Mucor pusillus.
Lipase of Mucor pusillus NRRL 2543 was recovered with ammonium sulfate precipitation, gel filtration on Sephadex G-75, and anion-exchange chromatography on diethylaminoethyl-Sephadex A-50. Maximal glycerol ester hydrolase (lipase) activity was observed at pH 5.0 to 5.5 and 50 C when trioctanoin and olive oil were used as substrates. The enzyme also showed esterase activity; it hydrolyzed, with ...
متن کاملIsolation, Identification and Characterization of Lipase Producing Microorganisms from Environment
Objective: Lipases represent an important group of hydrolytic proteins. These enzymes find numerous applications in the industrial sector to provide regioselective molecules of commercial interest. The present investigation was carried out to isolate and identify a bacterium that can quickly produce this enzyme from very simple and cost effective nutrient medium. Materials and Methods: Lipid as...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2000
ISSN: 1028-8880
DOI: 10.3923/pjbs.2000.511.512